New progress in
biocompatible and bioactive nanostructures
synthesized by pulsed laser systems
C. Ristoscu1,
P. Torricelli2, A. Bigi3, I. Mayer4, I.
N. Mihailescu1, M. Iliescu5, J. Werckmann5,
G. Socol1, F. Cuisinier6, R. Elkaim6,
G. Hildebrand7
1National
Institute for Lasers, Plasma and Radiation Physics, 76900
Bucharest-Magurele, Romania
2Servizio
di Chirurgia Sperimentale-Istituto di Ricerca Codivilla PuttiIOR,
Bologna, Italy
3Department
of Chemistry "G. Ciamician",
University of Bologna, 40126 Bologna, Italy
4Department
of Inorganic and Analytical Chemistry, Hebrew University of Jerusalem,
91904, Jerusalem, Israel
5Institute
of Physics and Chemistry of Materials, 67037 Strasbourg, France
6Institut
National de la Santé et de la Recherche Médicale, 67085 Strasbourg,
France
7IBA
e.V., Department of Biomaterials, Rosenhof, D-37308 Heilbad
Heiligenstadt, Germany
We
extended for the first time pulsed laser ablation to the deposition of
octacalcium phosphate Ca8H2(PO4)6·5H2O
(OCP) and hydroxyapatite, Ca10(PO4)6(OH)2,
carbonated and doped with Mn2+ (HA:Mn) thin films containing
naostructured domains. Coatings were obtained by Pulsed Laser Deposition
technique, with a KrF* excimer laser source (λ
= 248 nm, τFWHM
≥
20 ns). The targets were sintered from the respective crystalline
powders and the laser fluence was set at values of 1.5 - 2 J/cm2.
During depositions, the collectors, Si or Ti substrates, were maintained
at a constant temperature within the range RT - 400°C. The resulting
structures were submitted to heat treatment in hot water vapors for up
to 6 h. High-resolution electron microscopy and XRD at grazing incidence
indicated that the coatings obtained consist of nanocrystalline
material.
The
investigations were optimized in obtaining structures fully
biocompatible and bioactive, as proved by the following in-vitro
tests: WST-1 assay, cell adherence, DNA replication, and caspase-1
activity confirmed the good biocompatibility of the coatings. Complete
in-vivo tests are in progress.